- 商品名称: TSKgel PW系列色谱柱
TSKgel PW系列色谱柱由亲水性球形聚甲基丙烯酸脂颗粒填充,填料粒径覆盖从12µm(小孔径色谱柱)到17µm(大孔径色谱柱)。 TSKgel PW系列色谱柱的主要应用范围在于分析水溶性聚合物,如纤维素、丙烯酰胺、二醇、葡聚糖、和低聚糖等。
TSKgel PW系列色谱柱由亲水性球形聚甲基丙烯酸脂颗粒填充,填料粒径覆盖从12µm(小孔径色谱柱)到17µm(大孔径色谱柱)。
TSKgel PW系列色谱柱的主要应用范围在于分析水溶性聚合物,如纤维素、丙烯酰胺、二醇、葡聚糖、和低聚糖等。
左上图为所有PW型柱的校准曲线。不同聚合物样品的适用溶剂请详见下表。
TSKgel PW型特制柱
TSKgel GMPW,为混合型色谱柱,具有更宽范围的的线性校准范围,适合于MW分布较宽的样品和分子量未知的样品。 TSKgel G2000PW的粒径径较大(与TSKgel G-Oligo-PW相等),低聚糖的半制备和制备分离。 TSKgel G1000PW,是低分子量低聚物分析的。
聚合物类型 | 典型样品 | 适合的溶剂 |
非离子亲水型 | 聚乙二醇 | 蒸馏水 |
非离子疏水型 | 聚乙烯吡咯烷酮 | 含有有机溶剂的缓冲液或盐溶液( 例如:0.1 mol/L NaNO3中添加20% CH3CN ) |
阴离子亲水性 | 钠、藻酸钠、羧甲基纤维素、聚丙烯酸钠、透明质酸钠 | 缓冲液或盐溶液 (例如:0.1 mol/L NaNO3) |
阴离子疏水 | 磺化木质素钠盐、聚苯乙烯磺酸钠 | 含有有机溶剂的缓冲液或盐溶液( 例如:0.1 mol/L NaNO3中添加20% CH3CN) |
阳离子亲水性 | 乙二醇壳聚糖、DEAE-葡聚糖、聚乙烯亚胺、聚三甲基氨基乙基甲基丙烯酸脂、碘化盐 | 含有0.3 mol/L Na2SO4 的0.5M乙酸或0.8 mol/L NaNO3 |
阳离子疏水 | 聚4-乙烯基苄基三甲基氯化铵、聚N-甲基-2-乙烯基吡啶、碘化盐 | 含有0.3 mol/L Na2SO4的0.5 mol/L 乙酸 |
两性亲水性 | 肽、蛋白质、多糖和寡糖、DNA、RNA | 缓冲液和盐溶液( 例如:0.1 mol/L NaNO3) |
两性疏水 | 蓝色葡聚糖、胶原、明胶、疏水性蛋白、疏水性肽 | 含有有机溶剂的缓冲液或盐溶液 (例如:0.1 mol/L NaNO3 中添加20% CH3CN或0.1% TFA中添加35-45% CH3CN) |
色谱柱: | TSKgel G2000PW | TSKgel G2500PW | TSKgel G3000PW | TSKgel G4000PW |
基质: | 聚甲基丙烯酸脂 | 聚甲基丙烯酸脂 | 聚甲基丙烯酸脂 | 聚甲基丙烯酸脂 |
粒径: | 12 µm | 12 µm and 17 µm | 12 µm and 17 µm | 17 µm |
孔径: | 12.5 nm | <20 nm | 20 nm | 50 nm |
分离范围 (聚环氧乙烷): | up to 2,000 Da | up to 3,000 Da | up to 50,000 Da | up to 300,000 Da |
色谱柱: | TSKgel G4000PW | TSKgel G5000PW | TSKgel G6000PW | TSKgel GMPW |
基质: | 聚甲基丙烯酸脂 | 聚甲基丙烯酸脂 | 聚甲基丙烯酸脂 | 聚甲基丙烯酸脂 |
粒径: | 17 µm | 17 µm | 17 µm | 17 µm |
孔径: | 50 nm | 100 nm | >100 nm | <10-100 nm |
分离范围 (聚环氧乙烷): | up to 300,000 Da | up to 1,000,000 Da | up to 8,000,000 Da | 500 to 8,000,000 Da |
规格型号
P/N | Description | Particle size | Housing material | ID (mm) | Length (cm) |
0005761 | TSKgel G2000PW | 12 µm | Stainless Steel | 7.5 | 30 |
0005105 | TSKgel G2000PW | 12 µm | Stainless Steel | 7.5 | 60 |
0006763 | Guard Column for P/N 0005761 and 0005105 | 12 µm | Stainless Steel | 7.5 | 7.5 |
0008028 | TSKgel G2500PW | 12 µm | Stainless Steel | 7.5 | 30 |
0008029 | TSKgel G2500PW | 12 µm | Stainless Steel | 7.5 | 60 |
0006762 | Guard Column for P/N 0008028, 0008029 | 12 µm | Stainless Steel | 7.5 | 7.5 |
0016248 | TSKgel G2500PW | 17 µm | Stainless Steel | 21.5 | 30 |
0008030 | TSKgel G2500PW | 17 µm | Stainless Steel | 21.5 | 60 |
0006758 | Guard Column for P/N 0016248, 0008030 | 17 µm | Stainless Steel | 21.5 | 7.5 |
0005762 | TSKgel G3000PW | 12 µm | Stainless Steel | 7.5 | 30 |
0005106 | TSKgel G3000PW | 12 µm | Stainless Steel | 7.5 | 60 |
0006762 | Guard Column for P/N 0005762, 0005106 | 12 µm | Stainless Steel | 7.5 | 7.5 |
0016249 | TSKgel G3000PW | 17 µm | Stainless Steel | 21.5 | 30 |
0005151 | TSKgel G3000PW | 17 µm | Stainless Steel | 21.5 | 60 |
0006758 | Guard Column for P/N 0016249, 0005151 | 17 µm | Stainless Steel | 21.5 | 7.5 |
0005763 | TSKgel G4000PW | 17 µm | Stainless Steel | 7.5 | 30 |
0005107 | TSKgel G4000PW | 17 µm | Stainless Steel | 7.5 | 60 |
0006762 | Guard Column for P/N 0005763, 0005107 | 12 µm | Stainless Steel | 7.5 | 7.5 |
0016250 | TSKgel G4000PW | 20 µm | Stainless Steel | 21.5 | 30 |
0005152 | TSKgel G4000PW | 20 µm | Stainless Steel | 21.5 | 60 |
0006758 | Guard Column for P/N 0016250, 0005152 | 17 µm | Stainless Steel | 21.5 | 7.5 |
0005764 | TSKgel G5000PW | 17 µm | Stainless Steel | 7.5 | 30 |
0005108 | TSKgel G5000PW | 17 µm | Stainless Steel | 7.5 | 60 |
0006762 | Guard Column for P/N 0005764, 0005108 | 12 µm | Stainless Steel | 7.5 | 7.5 |
0016251 | TSKgel G5000PW | 20 µm | Stainless Steel | 21.5 | 30 |
0005153 | TSKgel G5000PW | 20 µm | Stainless Steel | 21.5 | 60 |
0006758 | Guard Column for P/N 0016251, 0005153 | 17 µm | Stainless Steel | 21.5 | 7.5 |
0005765 | TSKgel G6000PW | 17 µm | Stainless Steel | 7.5 | 30 |
0005109 | TSKgel G6000PW | 17 µm | Stainless Steel | 7.5 | 60 |
0006762 | Guard Column for P/N 0005765 and 0005109 | 12 µm | Stainless Steel | 7.5 | 7.5 |
0020024 | TSKgel BioAssist G6PW | 17 µm | PEEK | 7.8 | 30 |
0008026 | TSKgel GMPW | 17 µm | Stainless Steel | 7.5 | 30 |
0008027 | TSKgel GMPW | 17 µm | Stainless Steel | 7.5 | 60 |
0006762 | Guard Column for P/N 0008026 and 0008027 | 12 µm | Stainless Steel | 7.5 | 7.5 |